Objective

The course is focused on experimental and theoretical methods to study how the brain operates at the level of neuronal circuits. We cover various optical and electrophysiological concepts and techniques used currently in systems neuroscience from the basics to advanced topics on both theoretical and experimental grounds.

The course is designed to be a highly interactive, hands-on experience, reflecting the atmosphere of CSHL, Woods Hole or Champalimaud courses. Typically, each course day will contain an extended lab session and several theoretical lectures. Hard work will be combined with a few trips through the beautiful Transylvanian countryside. The course is addressed to a graduate student/postdoc audience.

Program coming soon.
  See last year's program for more details.


Lecturers

  • Athena Akrami – Sainsbury Wellcome Centre, UCL, UK
  • Upinder Bhalla – National Centre for Biological Sciences, India
  • Federico Carnevale – DeepMind Technologies, London, UK
  • Florian Engert – Harvard University, USA
  • Balázs Hangya – IEM, Hungarian Academy of Sciences, Hungary
  • Sonja Hofer – Sainsbury Wellcome Centre, University College London, UK
  • TomᚠHromαdka – Slovak Academy of Sciences, Slovakia
  • Benjamin Judkewitz – Einstein Center for Neuroscience, Germany
  • Georg Keller – Friedrich Miescher Institute, Switzerland
  • Christian Machens – Champalimaud Foundation, Portugal
  • MacKenzie Mathis – Rowland Institute, Harvard University
  • Marta Moita – Champalimaud Foundation, Portugal
  • Hannah Monyer – DKFZ, University of Heidelberg, Germany
  • Tom Mrsic-Flögel – Sainsbury Wellcome Centre, University College London, UK
  • Venkatesh Murthy – Harvard University, USA
  • Marius Pachitariu – HHMI, Janelia Research Campus, USA
  • Ruben Portugues – Max Planck Institute of Neurobiology, Germany
  • Tobias Rose – Max Planck Institute of Neurobiology, Germany
  • Botond Roska – Friedrich Miescher Institute, Switzerland
  • Wolf Singer – Max Planck Institute for Brain Research, Germany
  • Ramesh Srinivasan – University of California Irvine, USA
  • Nao Uchida – Harvard University, USA
  • Daniela Vallentin – Max-Planck-Institute for Ornithology, Germany
  • Mitsuko Watabe-Uchida – Harvard University, USA
  • Chris Xu – Cornell University, USA


Organizers

  • Florin Albeanu – Cold Spring Harbor Laboratory, NY, USA
  • Adam Kampff – Sainsbury Wellcome Centre, University College London, UK
  • Raul Mureşan – Transylvanian Institute of Neuroscience, Cluj-Napoca, Romania

Teaching Assistants

  • Harald Bârzan – Transylvanian Institute of Neuroscience, Cluj-Napoca, Romania
  • Antonin Blot – Sainsbury Wellcome Centre, UCL, UK
  • Rob Campbell – Sainsbury Wellcome Centre, UCL, UK
  • Andrei Ciuparu – Transylvanian Institute of Neuroscience, Cluj-Napoca, Romania
  • Aleena Garner – Friedrich Miescher Institute, Basel, Switzerland
  • Medorian Gheorghiu – Transylvanian Institute of Neuroscience, Cluj-Napoca, Romania
  • Priyanka Gupta – Cold Spring Harbor Laboratory, NY, USA
  • Mitra Javadzadeh – Sainsbury Wellcome Centre, UCL, UK
  • Mateusz Kostecki – Nencki Institute for Experimental Biology, Warsaw, Poland
  • Gonçalo Lopes – Sainsbury Wellcome Centre, UCL, UK
  • Fred Marbach – Sainsbury Wellcome Centre, UCL, UK
  • Vasile V. Moca – Transylvanian Institute of Neuroscience, Cluj-Napoca, Romania
  • Mehrab Modi – Janelia Research Campus, USA
  • Adriana Nagy-Dăbâcan – Transylvanian Institute of Neuroscience, Cluj-Napoca, Romania
  • Jon Newman – Massachusetts Institute of Technology, USA
  • Bruno Pichler – INSS (Independent NeuroScience Services), UK
  • Nacho Sanguinetti – Bernstein Center for Computational Neuroscience, Berlin, Germany
  • Hande Tunbak – Wolfson Institute of Biomedical Research - Division of Medicine, UCL, UK
  • Iuliu Vasilescu – Politechnica University, Bucharest, Romania
  • Jakob Voigts – Massachusetts Institute of Technology, USA
  • Petr Znamenskiy – Sainsbury Wellcome Centre, UCL, UK

Support and administration

  • Adrian Ardelean – EFSA EU FORA Fellow, Italy
  • Cosmina Pavel – Transylvanian Institute of Neuroscience, Cluj-Napoca, Romania
  • Attila Kelemen – Babes-Bolyai University, Cluj-Napoca, Romania

Topics

  • Basic Optics – Diffraction and Resolution. Illumination Techniques. Numerical Aperture.
  • Optical bench exercises – Lenses, optical systems, illumination methods, basic microscopy techniques. How to custom build different kinds of microscopes.
  • Noise measurements and photo-sensors – Shot noise, optical detectors, amplifiers, NI-DAQ, CCD cameras, photodiodes, photo multiplier tubes (PMTs).
  • Light and fluorescence microscopy – Fluorescence, FRAP, photo-activation, photo-conversion. Point spread function measurements, basic image analysis (deconvolution, denoising, PCA).
  • Fluorescence probes – GFP, GFP based chromophores, organic calcium dyes, genetically encoded calcium dyes, pHluorins, voltage sensitive dyes.
  • Intrinsic Optical Imaging – Visual, auditory & barrel cortex; olfactory bulb. Students will build a custom wide field fluorescence and intrinsic optical imaging rig.
  • Scanning microscopy – Confocal and two-photon microscopy. Lasers. Students will build a two-photon microscope and write custom scanning and acquisition software in MATLAB and NI DAQmx. The ScanImage API.
  • Viral approaches to label, monitor and alter neuronal circuits.
  • Optogenetics – Light activated ion channels and pumps. Patterned photo-stimulation techniques.
  • Benchtop electronics and basic electrophysiology – Impedence and Dipoles. Amplifiers. Extracellular and intracellular recordings. LFP; single unit, multi-unit extracellular recordings, tetrodes, electrode arrays; patch clamp.
  • Awake head fixed and freely moving optical and electrophysiological recording strategies in rodents – Microdrives. Fiber optic based systems. Open source systems. Open Ephys.
  • Techniques for electrophysiological data analysis.
  • Monitoring animal behavior – Open Source tools for acquisition and analysis of video data. Intro to Bonsai and Arduino. Training Strategies. Closed loop systems.
  • Neuronal functional connectivity and neuronal connectomics – Serial electron-microscopy and trans-synaptic labeling methods.
  • Synchrony and oscillations.
  • Cortical attention, sparse neuronal codes.
  • Decision making, uncertainty, neuro-modulatory systems.

© TENSS 2019